A collection of our most frequently used protocols. Please reference the appropriate papers (listed below) when using protocols from this page.
Alexandra M. Tayar, Linnea M. Lemma, Zvonimir Dogic
Reference
"Preparation of Modified Tubulins," Anthony Hyman, David Dreschel, Doug Kellogg, Steve Salser, Ken Sawin, Pam Steffen, Linda Wordeman, Tim Mitchison, Methods in Enzymology 196, 478-485 (1991). [Web]
Reference
"Preparation of Modified Tubulins," Anthony Hyman, David Dreschel, Doug Kellogg, Steve Salser, Ken Sawin, Pam Steffen, Linda Wordeman, Tim Mitchison, Methods in Enzymology 196, 478-485 (1991). [Web]
Reference
"Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer," Mirco Castoldi, Andrei V. Popov, Protein Expression and Purification 32, 83-86 (2003). [Web]
Reference
"Condensation of isolated semi-flexible filaments driven by depletion interactions," A. W. C. Lau, A. Prasad and Z. Dogic EPL 87, 48006 (2009). [PDF]
Reference
"Efficient one-step PEG-silane passivation of glass surfaces for single-molecule fluorescence studies," Y. Gidi, S. Bayram, C. J. Ablenas, A. S. Blum, and G. Cosa, ACS Appl. Mater. Interfaces 10, 46 (2018). [Web]
Reference
"Orientational order of motile defects in active nematics," Stephen J. Decamp, Gabriel S. Redner, Aparna Baskaran, Michael. F. Hagan and Zvonimir Dogic, Nature Materials, 14, 1110-1115 (2015). [PDF]
Reference
"Condensation of isolated semi-flexible filaments driven by depletion interactions," A. W. C. Lau, A. Prasad and Z. Dogic EPL 87, 48006 (2009). [PDF]
Reference
"Microtubule Movement by a Biotinated Kinesin Bound to a Streptavidin-coated Surface," E. Berliner, H. K. Mahtani, L. F. Chu, J. E. Cronan Jr., J. Gelles Journal of Biological Chemistry 269, 8610-8615 (1993). [PDF]
Reference
"Microtubule Movement by a Biotinated Kinesin Bound to a Streptavidin-coated Surface," E. Berliner, H. K. Mahtani, L. F. Chu, J. E. Cronan Jr., J. Gelles Journal of Biological Chemistry 269, 8610-8615 (1993). [PDF]